Development of metronidazole-resistant lines of Blastocystis sp.
Identifieur interne : 005D29 ( Main/Exploration ); précédent : 005D28; suivant : 005D30Development of metronidazole-resistant lines of Blastocystis sp.
Auteurs : L. A. Dunn [Australie] ; K. S. W. Tan [Singapour] ; P. Vanelle [France] ; T. Juspin [France] ; M. D. Crozet [France] ; T. Terme [France] ; P. Upcroft [Australie] ; J. A. Upcroft [Australie]Source :
- Parasitology research : (1987) [ 0932-0113 ] ; 2012.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Metronidazole (MTR) is frequently used for the treatment of Blastocystis infections, but with variable effectiveness, and often with treatment failures as a possible result of drug resistance. We have developed two Blastocystis MTR-resistant (MTRR) subtype 4 WR1 lines (WR1-M4 and WR1-M5), with variable susceptibility to a panel of anti-protozoal agents including various 5-nitroimidazoles, nitazoxanide and furazolidone. WR1-M4 and WR1-M5 were developed and assessed over an 18-month period and displayed persistent MTR resistance, being more than 2.5-fold less susceptible to MTR than the parent isolate. The MTRR lines grew with a similar g time to WR1, but were morphologically less consistent with a mixture of size. All Blastocystis isolates and the MTRR lines were most susceptible to the 5-nitroimidazole drug ronidazole. WR1-M5 was apparently cross-resistant to satranidazole and furazolidone, and WR1-M4 was cross-resistant to nitazoxanide. These MTRR lines now provide a valuable tool for the continued assessment of the efficacy and mechanism of action of new and established drugs against a range of Blastocystis sp. subtypes, in order to identify a universally effective drug and to facilitate understanding of the mechanisms of drug action and resistance in Blastocystis.
Affiliations:
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A." last="Dunn">L. A. Dunn</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland Institute of Medical Research, PO Royal Brisbane Hospital</s1><s2>4029 Herston, Queensland</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>4029 Herston, Queensland</wicri:noRegion></affiliation></author><author><name sortKey="Tan, K S W" sort="Tan, K S W" uniqKey="Tan K" first="K. S. W." last="Tan">K. S. W. Tan</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore</s1><s2>Singapore</s2><s3>SGP</s3><sZ>2 aut.</sZ></inist:fA14><country>Singapour</country><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Vanelle, P" sort="Vanelle, P" uniqKey="Vanelle P" first="P." last="Vanelle">P. 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Juspin</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Laboratoire de Pharmaco-Chimie Radicalaire, Faculté de Pharmacie, Universites d'Aix-Marseille I, II, III-CNRS, UMR 6264: Laboratoire Chimie Provence</s1><s2>Marseille</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Crozet, M D" sort="Crozet, M D" uniqKey="Crozet M" first="M. D." last="Crozet">M. D. Crozet</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Laboratoire de Pharmaco-Chimie Radicalaire, Faculté de Pharmacie, Universites d'Aix-Marseille I, II, III-CNRS, UMR 6264: Laboratoire Chimie Provence</s1><s2>Marseille</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Terme, T" sort="Terme, T" uniqKey="Terme T" first="T." last="Terme">T. Terme</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Laboratoire de Pharmaco-Chimie Radicalaire, Faculté de Pharmacie, Universites d'Aix-Marseille I, II, III-CNRS, UMR 6264: Laboratoire Chimie Provence</s1><s2>Marseille</s2><s3>FRA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Upcroft, P" sort="Upcroft, P" uniqKey="Upcroft P" first="P." last="Upcroft">P. Upcroft</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland Institute of Medical Research, PO Royal Brisbane Hospital</s1><s2>4029 Herston, Queensland</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>4029 Herston, Queensland</wicri:noRegion></affiliation></author><author><name sortKey="Upcroft, J A" sort="Upcroft, J A" uniqKey="Upcroft J" first="J. A." last="Upcroft">J. A. Upcroft</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland Institute of Medical Research, PO Royal Brisbane Hospital</s1><s2>4029 Herston, Queensland</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>4029 Herston, Queensland</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Parasitology research : (1987)</title><title level="j" type="abbreviated">Parasitol. res. : (1987)</title><idno type="ISSN">0932-0113</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Parasitology research : (1987)</title><title level="j" type="abbreviated">Parasitol. res. : (1987)</title><idno type="ISSN">0932-0113</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Development</term><term>Metronidazole</term><term>Parasite</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Développement</term><term>Métronidazole</term><term>Parasite</term><term>Blastocystis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metronidazole (MTR) is frequently used for the treatment of Blastocystis infections, but with variable effectiveness, and often with treatment failures as a possible result of drug resistance. We have developed two Blastocystis MTR-resistant (MTR<sup>R</sup>) subtype 4 WR1 lines (WR1-M4 and WR1-M5), with variable susceptibility to a panel of anti-protozoal agents including various 5-nitroimidazoles, nitazoxanide and furazolidone. WR1-M4 and WR1-M5 were developed and assessed over an 18-month period and displayed persistent MTR resistance, being more than 2.5-fold less susceptible to MTR than the parent isolate. The MTR<sup>R</sup> lines grew with a similar g time to WR1, but were morphologically less consistent with a mixture of size. All Blastocystis isolates and the MTR<sup>R</sup> lines were most susceptible to the 5-nitroimidazole drug ronidazole. WR1-M5 was apparently cross-resistant to satranidazole and furazolidone, and WR1-M4 was cross-resistant to nitazoxanide. These MTR<sup>R</sup> lines now provide a valuable tool for the continued assessment of the efficacy and mechanism of action of new and established drugs against a range of Blastocystis sp. subtypes, in order to identify a universally effective drug and to facilitate understanding of the mechanisms of drug action and resistance in Blastocystis.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Singapour</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Dunn, L A" sort="Dunn, L A" uniqKey="Dunn L" first="L. A." last="Dunn">L. A. Dunn</name></noRegion><name sortKey="Upcroft, J A" sort="Upcroft, J A" uniqKey="Upcroft J" first="J. A." last="Upcroft">J. A. Upcroft</name><name sortKey="Upcroft, P" sort="Upcroft, P" uniqKey="Upcroft P" first="P." last="Upcroft">P. Upcroft</name></country><country name="Singapour"><noRegion><name sortKey="Tan, K S W" sort="Tan, K S W" uniqKey="Tan K" first="K. S. W." last="Tan">K. S. W. Tan</name></noRegion></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Vanelle, P" sort="Vanelle, P" uniqKey="Vanelle P" first="P." last="Vanelle">P. Vanelle</name></region><name sortKey="Crozet, M D" sort="Crozet, M D" uniqKey="Crozet M" first="M. D." last="Crozet">M. D. Crozet</name><name sortKey="Juspin, T" sort="Juspin, T" uniqKey="Juspin T" first="T." last="Juspin">T. Juspin</name><name sortKey="Terme, T" sort="Terme, T" uniqKey="Terme T" first="T." last="Terme">T. Terme</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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